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Becton Dickinson
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Image Search Results
Journal: Scientific Reports
Article Title: CD4 + and CD8 + T Cells Exert Regulatory Properties During Experimental Acute Aristolochic Acid Nephropathy
doi: 10.1038/s41598-018-23565-2
Figure Lengend Snippet: Absolute number of specific kidney lymphocyte subpopulations per gram of kidney tissue ( a ). No statistical change in the absolute number of CD3 + , CD4 + or CD8 + lymphocytes was observed after AA injections (greys columns) as compared to CTRL group (white columns). However an increase in the percentage of splenic ( b ) and kidney ( c ) CD3 + CD4 + CD25 high FOXP3 + T-reg cells per CD3 + CD4 + T cells was observed after AA injection (greys columns) as compared to CTRL group (white columns). Statistical test used: Mann-Whitney. Results are expressed as the mean ± SEM, *p < 0.05; **p < 0.01. Number of mice per group: AA n = 14; CTRL n = 6.
Article Snippet: The fluorochrome-conjugated mAb to mouse antigens used for flow cytometry analysis were from eBioscience (VWR, Haasrode, Belgium): anti-CD16/CD32, anti-Ly-6c (PE conjugated; clone HK1.4), anti F4/80 (APC conjugated; clone BM8), anti-Foxp3 (APC conjugated; clone FJK-16s); from
Techniques: Injection, MANN-WHITNEY
Journal: Scientific Reports
Article Title: CD4 + and CD8 + T Cells Exert Regulatory Properties During Experimental Acute Aristolochic Acid Nephropathy
doi: 10.1038/s41598-018-23565-2
Figure Lengend Snippet: As illustrated on this representative CD11b-F4/80 dots plot, ATN lesions observed after AA injections were associated with a sharp decrease in the P2 population and a significant increase in the P1 population ( a ). Absolute number of neutrophils (NEU), CD11c+, P1 and P2 population per gram of kidney in AA + αCD4 (black columns), AA (dark greys columns), αCD4 (light greys columns) and CTRL (white columns) groups on day 5 ( b ). As compared to CTRL group, a non significant increase in the absolute number of CD11c + , a significant increase in the absolute number of P1 populations and a significant reduction in the number of P2 population were observed after AA injection. Moreover, in the AA + αCD4 the increase in CD11c + and P1 populations was even greater than those found in AA group. After CD8 depletion, a significant decrease in P2 population was also observed as compared to AA group ( c ). Statistical test used: ANOVA followed by t-test and Bonferroni correction for multiple comparisons or Mann-Whitney U-test for simple comparisons. Results are expressed as the mean ± SEM, ****p < 0.0001, **p < 0.01, *p < 0.05; NS = no statistical difference observed. Number of mice per group: AA + αCD4 n = 13; AA n = 14; αCD4 n = 9; AA + αCD8 n = 8; αCD8 n = 8; CTRL n = 6.
Article Snippet: The fluorochrome-conjugated mAb to mouse antigens used for flow cytometry analysis were from eBioscience (VWR, Haasrode, Belgium): anti-CD16/CD32, anti-Ly-6c (PE conjugated; clone HK1.4), anti F4/80 (APC conjugated; clone BM8), anti-Foxp3 (APC conjugated; clone FJK-16s); from
Techniques: Injection, MANN-WHITNEY